| Melting Point | 240°C (dec., lit.) |
| Color | White to off-white |
| Linear Formula | NH2CH2COOH |
| Formula Weight | 75.07 |
| Physical Form | Crystal-powder |
| Chemical Name or Material | Glycine [for Electrophoresis] |
| Molecular Weight | 75.07 g/mol |
| SMILES String | NCC(O)=O |
| Solubility Information | 200 mg/mL (water), clear |
| CAS Number | 56-40-6 |
| Storage Temperature | Room temperature |
| EC Number | 200-272-2 |
| NACRES | NA.26 |
| PubChem Substance ID | 24895290 |
| UNSPSC Code | 12352209 |
| MDL Number | MFCD00008131 |
| InChI | 1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5) |
| InChI Key | DHMQDGOQFOQNFH-UHFFFAOYSA-N |
| Beilstein/REAXYS Number | 635782 |
| Quality Level | 200 |
| Reference Grade | ReagentPlus®, ≥99% (HPLC), suitable for Western blotting |
| pKa (25°C) | 2.35 (1st), 9.60 (2nd) |
| Applications | SDS-PAGE and Western blotting buffer component (electrophoresis) |
Glycine is the smallest and only non-chiral proteinogenic amino acid, with an isoelectric point (pI) of 6.7 that happens to sit close to the pH of the stacking region in standard polyacrylamide gels. That property — combined with low protein-binding affinity and hydrophobicity — is exactly what makes glycine such an effective, low-interference component of SDS-PAGE and Western blot buffer systems.
Glycine’s most common laboratory role is as a buffering component in SDS-PAGE running buffers and Western blot transfer buffers, where its zwitterionic nature and low protein interaction support clean, reproducible protein separation and transfer. It’s also used in cell-culture media, in solutions for dissolving formazan crystals in MTT cytotoxicity assays, and as a reaction-terminating reagent in various enzymatic and cell-based assays.
*This reference listing carries no GHS hazard pictogram or hazard statement — it is not classified as GHS-hazardous.*
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